The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
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The solvent delivery system includes a pump, through which solvent (cellular phase) is delivered at a controlled circulation amount. If air will get dissolved during the mobile section, it may make air bubbles that fluctuate the flow fee.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
, which enables us to check out a wide number of cellular phases with only seven experiments. We get started by adjusting the quantity of acetonitrile within the mobile section to create the very best separation inside of the specified analysis time.
Unique solvents have various polarities, which impact their interaction Along with the stationary period and in the long run have an impact on the separation of analytes. Frequent solvents Utilized in HPLC involve:
A detector identifies and actions Just about every element. Retention time indicates enough time taken for every compound to exit the column. HPLC's performance is determined by things like column variety and cellular period composition. Standard upkeep guarantees accurate outcomes. Understanding HPLC's phase-by-action process is important for exact chemical Evaluation in laboratories.
In liquid–liquid chromatography the stationary stage is usually a liquid film coated over a packing product, typically 3–ten μm porous silica particles. As the stationary period might be partially soluble within the cellular stage, it may elute, or bleed in the column over time.
順相クロマトグラフィーは高速液体クロマトグラフィーにおいて最初に使われた。固定相に高極性のもの（シリカゲル）を、移動相に低極性のもの（例えばヘキサン、酢酸エチル、クロロホルムなどの有機溶媒）を用いる。分析物はより極性の高いほどより強く固定相と相互作用して溶出が遅くなる。また極性の高い物質の割合が多い移動相ほど溶出が早くなる。順相タイプは近年の逆相タイプの発展とともに使われることが少なくなったが、順相タイプは逆相タイプをはじめとする他の分離モードとは異なった特性を持つため、目的によっては非常に有効なものとなる。例えば、逆相タイプでは分離が困難なトコフェロールの異性体や保持の困難な糖類を容易に相互分析することができ、また主に水を含まない移動相を用いるので、水に難溶の脂溶性ビタミンや加水分解されやすい酸無水物などの化合物の分離に好適である。
Ghost peaks are extraneous peaks that surface while in the chromatogram but Do not correspond to any parts within the sample. These can complicate information Evaluation. Here are several prospective results in and solutions:
Ion-Trade chromatography is predicated around the more info separation of substances primarily based on their own charge. The stationary section incorporates billed groups that bring in and keep oppositely billed ions from your sample.
High-performance liquid chromatography is usually a modified and enhanced kind of column liquid chromatography and utilizes high tension. HPLC is Utilized in biochemistry and analytical chemistry. This method was produced in 1969 by Kirkland and Huber.
Compounds within the sample partition concerning the stationary stage plus the mobile period in partition chromatography. Compounds using a much better affinity for your stationary phase expend far more time interacting read more with it, causing slower elution from your column.
특히 컬럼의 선정은 분석의 결과에 영향을 미치기에 신중하게 선택하여야 합니다.
, and that is the more typical type of HPLC, the stationary section is nonpolar plus the cellular phase is polar. The commonest nonpolar stationary phases use an organochlorosilane wherever the R group is an n